Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

December 1st, 2023

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Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center, Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, ³Research Center of Genetic Resources, National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

* These authors contributed equally

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Yetişkin sineklerin sık sık taze gıda şişelerine aktarılmasına alternatif olarak Drosophila suşları için uzun süreli bir koruma yöntemi oldukça arzu edilir. Bu protokol, Drosophila primordial germ hücrelerinin dondurularak saklanmasını ve agametik konakçı embriyolara nakledilmeleri yoluyla suşun yeniden canlandırılmasını açıklar.

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Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

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