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Biology I: yeast, <em>Drosophila</em> and <em>C. elegans</em>
Larval Protein Staining in Drosophila
Description
Larval protein staining in Drosophila uses immunohistochemistry to show where proteins are located in tissues. This method relies on antibodies, which are proteins that bind specific epitopes, or small target regions, on the protein of interest. Because Drosophila larvae are easy to process and are transparent, some tissues ...
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Transcript
黑腹果蝇之所以能成为一种广泛研究的模式生物是因为它方便且广泛的应用。 果蝇幼虫的免疫组化是一门宝贵的技术，它能提供目的蛋白是否存在，定位以及与其它蛋白共定位等相关信息。 本短片将讲述果蝇幼虫组织的解剖，固定，封闭和封片等技术手段，以及它们的应用。
免疫组化是将交联了标记的的抗体来定位结合到特定的抗原蛋白使其可显色用于观察的一门技术。
果蝇幼虫免疫组化的成败首先取决于正确的解剖。由于幼虫组织十分脆弱，对它的解剖要求非常精准且迅速。 解剖通常在磷酸盐缓冲液PBS中进行。取出的组织先暂时置于PBS缓冲液中，其pH值与幼...
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