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HIGH SCHOOL

Biology

Science Experiments

Basic Biology

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Biology I: yeast, <em>Drosophila</em> and <em>C. elegans</em>

Isolating Nucleic Acids from Yeast

Description

One of the many advantages to using yeast as a model system is that large quantities of biomacromolecules, including nucleic acids (DNA and RNA), can be purified from the cultured cells.

This video will address the steps required to carry out nucleic acid extraction. We will begin by briefly outlining the growth and harvest, and lysis of ...

Transcript

Today we will be showing you how to purify nucleic acids from Saccharomyces cerevisiae, also known as baker’s yeast. Nucleic acids can include DNA or RNA. This video will discuss the isolation of these molecules from yeast cells by phase separation and chromatography.

Though many differ...

Tags

Nucleic Acid ExtractionYeast Cell LysisColumn BindingPhase SeparationSilica ColumnSpheroplast FormationGlass Bead HomogenizationMicroarray AnalysisGene Expression QuantificationRecombinant Protein Purification

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