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Developmental Biology

Reprogramming Cells into iPSCs

Description

Induced pluripotent stem cells, or iPSCs, are made by reprogramming mature somatic cells back into an undifferentiated state. These cells behave like embryonic stem cells in culture. Under the right growth conditions, they can then be directed to become different cell types.

This makes iPSCs an i...

Transcript

诱导性多能干细胞与人类胚胎干细胞类似,能够分化为体内几乎所有类型的细胞,因此在再生医学领域具有广阔的应用前景。

人类胚胎干细胞(hESCs)来源于着床前的胚胎,而诱导性多能干细胞(iPSCs)则是由完全分化的体细胞重编程获得。

在本视频中,您将学习生成iPSCs的基本原理、将已分化细胞诱导为多能状态的逐步实验方案,以及该技术的多种下游应用和改进方法。

首先,让我们讨论一下从体细胞类型生成诱导多能干细胞(iPSCs)的基本原理。

已分化的细胞,例如皮肤细胞或神经元,是命运已经确定的细胞,它们被限定执行特定功能。另一方面,多能干细胞...

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