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HIGH SCHOOL

Biology

Science Experiments

Advanced Biology

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Cell Biology

Watching Mitosis in Living Cells

Description

Mitosis is the process of cell division that splits a cell’s genetic material equally into two daughter cells. It moves through six phases, and each phase has its own visible changes in the chromosomes and other cell parts. Fluorescence live cell imaging lets scientists watch these changes in real time.

Transcript

有丝分裂是细胞周期中细胞核内容物高度有序且受控的分裂过程。有丝分裂对生物体的正常发育以及组织的生长、维持和修复至关重要。该过程的紊乱已被证实与某些疾病(如癌症)相关。目前,实验室研究有丝分裂最常用的方法之一是通过时间序列荧光显微镜进行活细胞成像。

本视频将简要介绍有丝分裂的各个阶段,然后讨论对该细胞过程进行活细胞成像时的实验设计要点。我们将展示详细的数据采集与分析流程,并最后介绍该技术的一些应用实例。

为了更好地理解科学家在这些成像实验中所关注的内容,我们首先来回顾一下有丝分裂的各个阶段。

细胞周期描述了细胞生长和分裂的整体过程。其中,有丝分裂期仅占该周期的一个较...

Tags

Mitosis PhasesFluorescent MicroscopyTime Lapse ImagingZ Stack AnalysisMontage Creation3D ReconstructionCell Cycle StagesSpindle Assembly CheckpointDNA Repair Foci

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