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Cell Biology
Tracking Internalized Membrane Proteins
Description
Cell-surface biotinylation assay is a method for tracking membrane proteins that move inside a cell through endocytosis. Endocytosis is the process cells use to take proteins from the membrane into the cytoplasm. Once inside, those proteins may be broken down or returned to the membrane by recycling.
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Transcript
Labeling of cell surface proteins with biotin presents a powerful tool to study cellular transport pathways involved in regulating membrane proteins. A cell maintains a tightly regulated set of proteins at the surface, so that it can receive and respond to extracellular information via several signaling pathways. Endocytosis, ...
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