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Advanced Biology

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Cell Biology

Cell Death Staining with Annexin V and PI

Description

Annexin V and propidium iodide (PI) staining helps identify how cells are dying. The method is used to tell apoptosis from necrosis. It does this by tracking two signals that change as a cell dies.

Annexin V is a protein that binds phosphatidylserine, a phospholipid that normally stays on the inn...

Transcript

Annexin V and propidium iodide (PI) labeling of cells is a technique used to identify cell death, and distinguish between its different pathways: apoptosis, or programmed cell death, and necrosis. Cells undergo distinct morphological changes depending on the pathway. By identifying the specific conditions that lead a cell to u...

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