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Cell Biology
Measuring Cell Movement with a Boyden Chamber
Description
Cell movement can be measured with a Boyden chamber, also called a transwell migration assay. Scientists use this setup to study how cells respond to chemical cues during development, immunity, and disease states such as cancer.
The chamber uses an insert that separates the wells of a multiwell p...
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Transcript
Transwell 迁移实验是一种经典技术,可使科研人员定量分析细胞运动。迁移是指细胞以单个或成簇方式移动的能力。细胞运动依赖于细胞骨架的精确重构,通常在受到作为信号刺激的诱导下发生。
今天我们将讨论 Transwell 迁移实验,该实验利用简单的腔室装置来评估细胞在趋化信号诱导下的迁移能力。
首先,我们将介绍一些关于Transwell小室的背景信息。
该装置最初由Stephen Boyden博士于1961年设计,用于研究白细胞迁移,因此该方法也被称为Boyden小室实验。
在简单的Boyden小室中,外壁为孔板的孔壁结构,类似...
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