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Research Article
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Erratum Notice
Important: There has been an erratum issued for this article. View Erratum Notice
Retraction Notice
The article Assisted Selection of Biomarkers by Linear Discriminant Analysis Effect Size (LEfSe) in Microbiome Data (10.3791/61715) has been retracted by the journal upon the authors' request due to a conflict regarding the data and methodology. View Retraction Notice
培养细胞的能力发生了革命性的基本细胞和分子生物学研究中的假设检验。它已经成为一个标准的方法,在药物筛选,毒理学和临床实验,并越来越多地使用再生医学。然而,传统的细胞培养方法,实质上是一个人口众多,在均匀的流体介质和细胞均匀的平面基板上的浸泡已经成为越来越多的限制,从根本和实践的角度都。微细加工技术使研究人员能够设计,用千分尺控制,生化成分和基板的拓扑结构,培养基成分,以及在邻近细胞周围的细胞微环境类型。此外,显微技术在概念上非常适合于发展快速,低成本的体外系统,使高通量条件下的大量细胞的培养和分析。伟业Folch在这次采访中,中解释了这些限制,如何将它们与软光刻技术和微流体克服,并介绍了一些相关的例子在他的实验室和未来发展方向的研究。