骨肉瘤 (OS) 的骨细胞外基质 (BEM) 模型已经建立, 并在此进行了展示。它可作为模拟原发肿瘤体外生长的合适支架, 为研究 os 的组织学和细胞生成异质性提供了理想的模型。
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
Access restricted. Please log in or start a trial to view this content.
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 15 mL 离心管 | Greiner | 188271 | |
| 50 mL 离心管 | Greiner | 227270 | |
| 6 cm 细胞培养皿 | Greiner | 628160 | |
| 6 孔板 | Greiner | 657160 | |
| 氨苄青霉素 | Sigma-Aldrich | A9393 | |
| C57-BL/6J 小鼠 | 中山大学实验室动物中心 | ||
| CO2 培养箱 | SHEL LAB | SCO5A | |
| 磷酸氢钠 | 广州化学试剂厂 | BE14-GR-500G | |
| DMEM/F12 | Sigma-Aldrich | D0547 | |
| 胎牛血清 | Hyclone | SH30084.03 | |
| 血细胞计数器 | BLAU | 717805 | |
| 卡那霉素 | Sigma-Aldrich | PHR1487 | |
| MG-63 | 中国科学院,上海细胞库 | 人骨肉瘤细胞系 | |
| MNNG/HOS | 中国科学院,上海细胞库 | 人骨肉瘤细胞系 | |
| 酚红 | Sigma-Aldrich | P4633 | 酚红溶液用作 pH 指示剂:其颜色在 6.6 至 8.0 的 pH 范围内逐渐从黄色转变为红色。 |
| 氯化钾 | Sangon Biotech | A100395 | |
| 磷酸钾 二氢磷酸钾 | Sangon Biotech | A501211 | |
| 氯化钠 | Sangon Biotech | A501218 |
Access restricted. Please log in or start a trial to view this content.
Request permission to reuse the text or figures of this JoVE article
Request Permission