In Vitro Gut Model
In Vitro Gut Model - Related Videos
02:12
In Vitro Batch-Culture Model to Mimic Colonic Fermentation by Human Gut Microbiota
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2025
Source: Ahmadi, S., et al. An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota. J. Vis. Exp. (2019).This video demonstrates a method for modeling gut microbial fermentation by incubating fecal samples with dietary fermentable fiber under anaerobic conditions to study metabolite production by gut microbiota.
02:44
In Vitro Gut Model to Assess Bacterial Adhesion and Internalization
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2026
Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018).This video demonstrates an in vitro gut host–microbe model to study bacterial viability. A co-culture of Caco-2 and HT29-MTX cells is incubated with bacteria pre-treated under simulated digestive conditions. Epithelial barrier integrity is assessed using transepithelial electrical resistance, and fractions from the medium, solubilized...
01:27
Oral Infection Alters the Gut Microbiota in a Rat Model
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2026
Begin with a rat model with sutures tied around its upper molar teeth to support bacterial colonization.Repeatedly smear the sutures with a gum disease-causing oral pathogenic bacterium using a gel-like solution.The bacteria colonize the gum around the sutures.Colonization triggers inflammation and damages the tissues.The bacteria release toxins that diffuse through damaged tissues and enter the bloodstream.These circulating toxins reach the gut, cause inflammation, disrupt the epithelial...
17:36
Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
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Cited by 11 •
2010
We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
FAQs
It applies food components, drugs, or biomaterials to sequential environments that represent major gastrointestinal stages. Conditions can be adjusted as the material moves through simulated stomach and intestinal settings, allowing researchers to examine how chemical exposure changes over time. This staged design supports controlled analysis of digestion, compound stability, and intestinal interactions without the variability of a whole-organism experiment.
These variables help determine how substances break down, remain stable, or interact with intestinal contents. Changes in pH alter the chemical environment, enzymes support digestion-related reactions, and transit represents movement through successive gastrointestinal regions. Controlling them separately or together helps researchers connect specific conditions with nutrient breakdown, drug behavior, or biomaterial performance.
Microorganisms add a biological dimension by allowing researchers to examine how microbial communities interact with intestinal contents. Their activity can contribute to metabolite production and influence the behavior of nutrients, drugs, or other compounds under intestinal conditions. Comparing systems with and without microorganisms can help distinguish chemical digestion from microbiome-associated effects in biochemical studies.
A study typically introduces a food component, drug, or biomaterial into controlled gastrointestinal conditions and then exposes it to sequential stomach and intestinal environments. Researchers monitor changes associated with digestion, stability, metabolite production, or microbial interaction. Because the conditions are controlled, the workflow can be used to compare substances or examine how individual variables influence biochemical outcomes.
Researchers may choose this approach when they need to isolate mechanisms and reduce experimental variability while studying gastrointestinal processes. It can support nutrition research, evaluation of drug delivery behavior, investigation of microbiome function, and analysis relevant to gastrointestinal disease. The controlled setting is especially useful for examining how defined chemical or biological conditions affect a test substance.
Results may reveal how nutrients are broken down, whether compounds remain stable across gastrointestinal conditions, and which metabolites are produced during intestinal or microbial activity. They can also clarify interactions between microbial communities and intestinal contents. In biochemistry, these measurements help connect changing gastrointestinal conditions with molecular transformations and support mechanistic interpretation of experimental findings.