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Bioengineering

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JoVE Journal Bioengineering
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Article DOI: 10.3791/61141-v • 08:25 min • June 5th, 2020
June 5th, 2020 •
1, 1, 1, 1, 1, 1, 1
1In vitro Toxicology Group, Swansea University Medical School

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Summary
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This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.

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3D Liver Models In Vitro Genotoxicity Testing Long-term Nanomaterial Exposure Hep G2 Model Viable And Functional Proliferation Biochemical Endpoints Genotoxicity Endpoints Micronucleus Assay Practice Runs Cell Seeding Changing Media 96 Well Culture Plate Sterile Room Temperature PBS Cell Suspension Multi-channel Pipette Spheroid Formation
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