Nanoparticle Production
Nanoparticle Production - Related Videos
09:01
A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating
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2016
This article presents a protocol for the production of protein-based nanoparticles that changes the hydrophobic surface to hydrophilic. The produced nanoparticle is an assembly of gliadin-cyanoacrylate diblock copolymers. Spray coating with the produced nanoparticle changes the surface of target material to a hydrophilic surface.
07:40
Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
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Cited by 14 •
2017
The antimicrobial properties of metals such as copper and silver have been recognized for centuries. This protocol describes pulsed laser-ablation in liquids, a method of synthesizing metal nanoparticles that provides the ability to fine tune the properties of these nanoparticles to optimize their antimicrobial effects.
06:02
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
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Cited by 14 •
2022
Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.
14:37
High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
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Cited by 7 •
2012
In this article, a high throughput method is presented for the synthesis of oligosaccharides and their attachment to the surface of polyanhydride nanoparticles for further use in targeting specific receptors on antigen presenting cells.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
FAQs
In bottom-up production, atoms, ions, or molecules first form nuclei, which then grow into larger nanoscale particles. The balance between nucleation and subsequent growth influences the resulting particle size and distribution. Controlling reaction conditions helps determine whether many small nuclei form or fewer particles grow further, making this mechanism important for reproducible material properties.
Top-down fabrication begins with a larger material and breaks it into nanoscale structures, whereas bottom-up fabrication assembles particles through nucleation and growth from smaller building units. These approaches control particle formation through different mechanisms. Comparing them helps researchers select a production strategy suited to the desired composition, structure, and level of control over nanoscale properties.
Stabilizing agents help limit aggregation, the unwanted joining of particles into larger clusters, while reaction conditions influence nucleation and growth. Together, these controls help maintain the intended particle size and other properties. Their use is especially important when consistent surface behavior or biological performance depends on preventing particles from changing during or after production.
Size, shape, surface chemistry, and composition can strongly affect how nanoparticles behave in biochemical systems. These features determine the characteristics of the particle interface and influence interactions with drugs, biomolecules, or sensing environments. Adjusting them allows researchers to connect nanoscale structure with biological function rather than treating all particles of the same material as equivalent.
A typical workflow selects a material and fabrication strategy, establishes reaction or processing conditions, and applies controls that limit aggregation and tune particle formation. The resulting product must then be characterized to determine whether its nanoscale structure matches the intended design. This sequence links fabrication decisions with measurable properties and later biochemical performance.
Characterization verifies the properties produced by the fabrication process, including features such as size, shape, surface chemistry, and composition. Without this information, researchers cannot reliably relate a particle's nanoscale structure to its observed biological or chemical behavior. Characterization therefore supports reproducibility and helps explain differences in delivery, sensing, imaging, or catalytic outcomes.
Biochemical researchers use produced nanoparticles as platforms for drug and biomolecule delivery, biosensing, imaging, and catalytic studies. Their high surface area and modifiable interfaces support interactions with biological molecules and experimental environments. Reliable fabrication and characterization are necessary to determine whether a particle's designed properties produce the intended function in these applications.