Journal
/
Biochemistry
/
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

DOI:
10.3791/62999-v

•

06:02 min

•

March 22, 2022

•

, , , , , ,
1Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering,Hokkaido University

Chapters

  • 00:04Introduction
  • 00:54Preparation of Lipid Solutions and the siRNA/ Buffer Solution
  • 01:58Set-Up of the iLiNP Device and Production of LNPs
  • 02:35Dialysis of the LNP Suspension and LNP Size Measurement
  • 03:24Results: POPC LNP Size Distributions Produced by the iLiNP Device at Different Flow Rate Ratios and Characterization and Cytotoxicity of siRNA-Loaded LNPs
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.

Tags

SiRNALipid NanoparticlesMicrofluidic DeviceSize ControlBiodistributionGene SilencingMRNARibonucleoproteinDOTAPDSPCCholesterolDMG-PEG2kSalineAcetate BufferSiGL4Dynamic Light ScatteringFlow RateFlow Rate Ratio

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Laboratory Scale Production and Purification of a Therapeutic Antibody

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Read Article