Bacteria Capture
Bacteria Capture - Related Videos
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
02:14
Prey Capture Assay: A Method to Study the Prey Capture Behavior of Zebrafish Larva
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2023
This video describes the prey capture assay. The video discusses the method to study prey capture behavior of zebrafish larvae after the laser ablation of specific neurons.
11:29
Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
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Cited by 52 •
2015
This video article describes experimental procedures to study long-term plasticity and its associative processes such as synaptic tagging, capture and cross-tagging in the CA1 pyramidal neurons using acute hippocampal slices from rodents.
10:58
Isolation of Fecal Bacteria from Water Samples by Filtration
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events include parasites, bacteria, and viruses that are shed in high numbers in the feces of infected people and animals. Transmission to new and susceptible hosts may...
14:04
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Cited by 17 •
2013
Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
FAQs
The capture mechanism determines selectivity. Size-based filtration retains cells according to physical dimensions, whereas antibody- or aptamer-coated surfaces and magnetic particles rely on molecular recognition of target bacteria. This distinction affects whether the method broadly concentrates bacterial cells or preferentially isolates a defined target for later detection, characterization, or removal.
Filtration physically retains bacterial cells from a liquid sample, while microfluidic systems guide small sample volumes through engineered flow paths that can support retention or recognition-based isolation. Both approaches can concentrate cells when their starting abundance is low, improving the amount of bacterial material available for microscopy, culture, nucleic acid testing, or biosensor analysis.
Antibodies and aptamers provide molecular recognition, allowing engineered surfaces to interact with selected bacterial targets rather than relying only on cell size. This targeted interaction supports selective isolation from a surrounding fluid. In bioengineering systems, the captured cells can then be examined or connected to downstream detection methods, including biosensors and nucleic acid testing.
Magnetic particles bind target bacterial cells through recognition-based interactions and enable the bound material to be separated from the surrounding fluid. Their role is therefore both capture and concentration. After separation, the enriched bacterial fraction can support detection or characterization, while the same principle can contribute to selective bacterial recovery or removal in engineered systems.
A typical workflow first exposes the sample to a retention or recognition system, such as a filter, microfluidic device, coated surface, or magnetic particle. The bacterial fraction is then isolated and concentrated from the surrounding fluid. The recovered material can proceed to culture, microscopy, nucleic acid testing, or biosensor-based analysis, depending on the objective.
Researchers may choose this approach when bacterial concentrations are low or when a sample requires selective recovery before analysis. Bioengineering applications include pathogen monitoring, diagnostics, environmental analysis, and bioprocess control. Capture can also support engineered systems intended to remove bacteria or recover selected cells, linking sample preparation with measurement or process management.