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JoVE Journal
Immunology and Infection

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T Cells Capture Bacteria by Transinfection from Dendritic Cells
 

T Cells Capture Bacteria by Transinfection from Dendritic Cells

Article doi: 10.3791/52976
January 13th, 2016

Summary January 13th, 2016

Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.

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