Bacterial Attachment Substrates

Source: Matthysse, A. G. Adherence of Bacteria to Plant Surfaces Measured in the Laboratory. J. Vis. Exp. (2018).This video demonstrates the evaluation of bacterial attachment to plant roots using phase-contrast microscopy. Root samples from seedlings incubated with bacteria are first mounted to check for free bacteria. The samples are then washed to remove loosely attached bacteria and remounted using a suitable coverslip to prevent damage. Finally, the number of bacteria attached to the root...

Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.

Attachment is vital for infant development, as warm social interactions support growth and well-being. In a classic 1958 study by Harry Harlow, the significance of warmth and comfort in forming attachments was examined. Harlow separated newborn monkeys from their mothers and provided two artificial "mothers": one made of cold wire and the other covered in soft cloth. Despite the wire mother offering food, the infant monkeys preferred the comfort of the cloth mother, demonstrating that physical...

Source: Fattahi, N., et al. Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation. J. Vis. Exp. (2021)This video demonstrates the use of photodegradable hydrogels to isolate bacterial colonies within silicon microwell arrays. Bacterial cells are first loaded into microwells, encapsulated in a crosslinked hydrogel, and cultured into distinct colonies. Upon exposure to ultraviolet light, targeted degradation of the hydrogel enables selective extraction of individual...

Jeffrey Simpson's attachment theory suggests that early caregiver relationships shape lasting patterns of behavior and emotional regulation, known as attachment styles. These patterns are organized along two key dimensions: self-esteem and interpersonal trust. The intersection of these dimensions produces four primary attachment styles that typically persist throughout life and significantly influence how individuals form and maintain relationships.Secure Attachment StyleIndividuals with a...