Myofibroblast Seeding
Myofibroblast Seeding - Related Videos
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
06:02
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
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Cited by 17 •
2016
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
08:59
Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
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Cited by 3 •
2015
Dupuytren’s disease (DD) is a fibroproliferative disease of the palm of the hand. Here, we present a protocol to culture resection specimens from DD in a three-dimensional (3D) culture system. Such short-term culture system allows preservation of the 3D structure and molecular properties of the fibrotic tissue.
02:33
Seed Structure and Early Development of the Sporophyte
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2020
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo. The embryo contains a double set of chromosomes, one set from each parent. Fertilization of the haploid egg by the haploid sperm gives rise to the zygote, which develops into the embryo. The endosperm is a...
11:18
Isolation of Myofibroblasts from Mouse and Human Esophagus
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Cited by 9 •
2015
We present a protocol to generate primary cultures of murine and human esophageal stromal cells with a myofibroblast phenotype. Cultured cells have spindle shaped morphology, express α-SMA and vimentin, and lack epithelial, hematopoietic and endothelial cell surface markers. Characterized stromal cells can be used in functional studies of epithelial-stromal interactions.
FAQs
Seeding density, substrate properties, and culture conditions are the main variables that shape the resulting model. Density can influence how cells organize and interact, while the substrate provides the physical context for attachment and force generation. Culture conditions then affect matrix deposition and remodeling. Controlling these variables is essential when comparing engineered tissues or repeating experiments.
After attachment, myofibroblasts spread across the culture surface and exert traction forces through their contractile behavior. Those forces act together with extracellular matrix production and remodeling, linking cell activity to tissue organization. This coupling matters because a model may reproduce cellular presence without reproducing the mechanical and structural features needed for meaningful bioengineering studies.
Reproducible myofibroblast seeding makes differences in tissue organization or contractility easier to attribute to scaffold or culture design rather than inconsistent cell introduction. It also supports physiologically relevant models by standardizing the starting cellular arrangement. In bioengineering, that consistency strengthens scaffold-performance evaluations and comparisons among engineered connective-tissue or vascular constructs.
A basic workflow begins by selecting a biomaterial, scaffold, or culture surface, then introducing the myofibroblasts under controlled conditions. The cells must attach and spread before their traction, matrix production, and remodeling can shape the model. Researchers then maintain the culture while considering seeding density, substrate properties, and other culture conditions that affect organization and contractility.
Useful outcomes include the degree of cell attachment and spreading, the organization of the developing tissue, extracellular matrix deposition and remodeling, and the resulting contractility. Together, these readouts show how cells interact with the selected material and whether the construct reflects relevant mechanical or structural features of native tissue. They can therefore guide scaffold evaluation and model refinement.
Myofibroblast seeding is especially relevant when a study needs connective-tissue or vascular models, because the cells contribute both contractile behavior and matrix-related activity. The approach also supports investigations of wound repair and fibrosis, where cell-matrix interactions and changes in tissue organization are important. These applications connect controlled culture experiments with engineered tissues designed to better reflect native tissue features.