Dissolved Oxygen Probe
Dissolved Oxygen Probe - Related Videos
09:38
Dissolved Oxygen in Surface Water
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Dissolved oxygen (DO) measurements calculate the amount of gaseous oxygen dissolved in surface water, which is important to all oxygen-breathing life in river ecosystems, including fish species preferred for human consumption (e.g. bluegill and bass), as well as decomposer species critical to the recycling of biogeochemical materials in the system. The oxygen dissolved in lakes, rivers, and oceans is crucial for the...
06:20
A Simple Approach to Manipulate Dissolved Oxygen for Animal Behavior Observations
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Cited by 1 •
2016
This article describes a simple and reproducible protocol to manipulate dissolved oxygen conditions in a laboratory setting for animal behavior studies. This protocol may be used in both teaching and research laboratory settings to evaluate organismal response of macroinvertebrates, fishes, or amphibians to changes in dissolved oxygen concentration.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
03:29
Flow Cytometry Assay to Quantify Oxidative Stress: An Assay to Quantify Reactive Oxygen Species in Intestinal Organoids Using Fluorogenic Probes
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2025
This video demonstrates a flow cytometry assay to quantify the ROS levels in 3D intestinal organoids obtained from GFP-expressing transgenic mice. This method provides an in vitro experimental model to investigate the efficacy of test compounds on ROS production.
FAQs
An electrochemical probe links oxygen transport to an electrical signal in two stages. Oxygen passes through the gas-permeable membrane and reaches the cathode, where reduction generates current. Greater oxygen availability changes that current, and calibration establishes how the signal corresponds to concentration. This conversion allows the instrument to report a quantitative value rather than only detecting oxygen presence.
Membrane transport and oxygen availability are central variables in the reading. Because the signal depends on oxygen reaching the cathode, changes in the liquid system’s oxygen availability are reflected in the measured current. In bioreactors and cultures, interpreting those changes can expose oxygen-transfer limitations, which helps distinguish a sensor reading from broader process behavior.
Calibration is essential because the probe does not directly display concentration from the cathode reaction alone. The measured current must be related to oxygen concentration before readings can be interpreted quantitatively. In bioengineering experiments, this step supports reliable comparisons across cell cultures, microbial systems, or bioreactor conditions and strengthens reproducibility when oxygen is used for monitoring or process control.
A practical workflow begins with calibration, followed by measurement of oxygen in the relevant liquid system. In a bioreactor, culture, or microbial setup, the resulting concentration reading can be monitored over time and used to maintain conditions that support growth and product formation. Comparing readings with process behavior also helps identify oxygen-transfer limitations and guide control decisions.
A single measurement principle serves two complementary needs. In water-quality work, the reading provides information about oxygen conditions in a liquid. In bioengineering, the same type of measurement supports observation and control of cell cultures, microbial systems, and bioreactors. This broad utility makes dissolved oxygen a common link between environmental analysis and engineered biological production.
They can indicate whether oxygen transfer is becoming a limiting part of the system, rather than merely documenting the current concentration. In cell cultures, microbial systems, and bioreactors, that information helps researchers adjust process conditions, evaluate support for growth, and understand effects on product formation. The measurements therefore contribute to process control and experimental reproducibility.