On-chip Assembly
On-chip Assembly - Related Videos
09:45
On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
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Cited by 9 •
2023
The present protocol describes octanol-assisted liposome assembly (OLA), a microfluidic technique to generate biocompatible liposomes. OLA produces monodispersed, micron-sized liposomes with efficient encapsulation, allowing immediate on-chip experimentation. This protocol is anticipated to be particularly suitable for synthetic biology and synthetic cell research.
11:13
Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Cited by 1 •
2016
We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
10:50
Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
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Cited by 16 •
2018
This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.
FAQs
Assembly is governed by the coordinated action of device geometry, surface patterning, fluid flow, and component interactions. Microfluidic channels direct cells, biomaterials, or other microscale elements, while patterned surfaces help establish where they remain. Physical or chemical interactions then support organization under defined conditions, allowing researchers to build structures with controlled spatial arrangement rather than relying on uncontrolled placement.
Defined fluid-flow conditions, surface patterns, and the physical or chemical interactions among components strongly influence organization. These factors determine how cells, biomaterials, or microscale elements are positioned and how consistently they assemble within the device. Controlling them is important because reproducible spatial arrangements support reliable studies of cell interactions, tissue organization, and biomaterial behavior.
Miniaturization provides tighter spatial control over the components being organized and can reduce reagent use. Because assembly occurs within a confined device, researchers can establish defined microenvironments for cells, biomaterials, or other microscale elements. These features make the approach useful when experiments require controlled organization, efficient material use, and reproducible observation of bioengineered structures.
A basic workflow begins by introducing the selected cells, biomaterials, or microscale components into a miniaturized device. Microfluidic channels and controlled flow then guide their movement, while patterned surfaces and physical or chemical interactions promote organization. Researchers perform these steps under defined conditions so the resulting structure can be examined for spatial arrangement and assembly behavior.
The approach supports construction of tissue models, organ-on-a-chip systems, biosensors, and other engineered microenvironments. Each application uses controlled placement and organization to create a system suited to a particular biological or engineering question. These platforms can reproduce selected features of tissue organization or provide arranged materials and cells for sensing and experimental analysis.
On-chip assembly creates reproducible settings for examining cell interactions, tissue organization, and biomaterial behavior. In bioengineering research, those organized microenvironments can support disease modeling, drug evaluation, and regenerative research. The value comes from linking controlled spatial construction with measurements or observations of how biological components and materials behave within an engineered system.