In Vitro Release
In Vitro Release - Related Videos
11:03
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
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Cited by 46 •
2016
High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.
01:10
In Vitro Drug Release Testing: Overview, Development and Validation
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2025
In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
05:39
Humanized Mediator Enzyme Release Assay: An Immunochemical Method to Screen Allergic Potential of Test Antigen by Monitoring Basophil Degranulation Response In Vitro
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2025
In this video, we describe an in vitro method to evaluate the allergic potential of an antigen to induce basophil degranulation. β-hexosaminidase is used as an index of chemical mediator release, and the enzyme activity of β-hexosaminidase is measured using a fluorogenic substrate.
01:28
Energy-releasing Steps of Glycolysis
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2019
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase. The first energy-releasing step—the 6th step of glycolysis —consists of two...
FAQs
Release behavior reflects the balance among diffusion, dissolution, swelling, and degradation of the carrier or device. These mechanisms can act alone or together, so a compound may leave a hydrogel, microsphere, nanoparticle, or implant at a different rate even when the same active compound is tested. Identifying the dominant process helps explain the resulting release profile and guides design decisions.
Temperature, agitation, and medium composition can influence the measured release profile by changing the conditions surrounding the delivery system. Because these variables are controlled experimental choices, differences between studies may reflect test conditions as well as formulation design. Reporting and maintaining them consistently is therefore important when evaluating performance, comparing formulations, or assessing quality.
A release profile shows how measured compound concentration changes over time, allowing investigators to compare the behavior of hydrogels, microspheres, nanoparticles, implants, and other devices. Differences in the profile can indicate that system structure or material behavior is affecting transport. This comparison supports platform optimization and helps assess whether formulations perform consistently.
A basic workflow begins by placing the delivery system in a selected release medium under controlled conditions. Samples are then collected at defined points over time, and the active compound concentration is quantified. Organizing these measurements into a release profile makes the time-dependent behavior visible and provides a basis for comparing formulations or evaluating the effect of test conditions.
Applications include hydrogels, microspheres, nanoparticles, implants, and other medical devices or biomaterials. Testing these different formats helps researchers characterize how each platform releases an active compound under laboratory conditions. The resulting data can support formulation optimization and quality assessment across a range of controlled-release designs, rather than limiting evaluation to one material type.
These studies generate early evidence about delivery performance before in vivo testing. Researchers can use the measured release profile to identify promising formulations, examine whether changes in temperature, agitation, or medium composition affect results, and support quality assessment. This role makes the method useful for narrowing design choices before advancing a drug delivery system or biomaterial to later evaluation.