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JoVE Journal
Neuroscience

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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
 

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Article DOI: 10.3791/58421
November 2nd, 2018

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Summary November 2nd, 2018

This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

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