Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

This content is Open Access.

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
 

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Article DOI: 10.3791/58421 10:50 min November 2nd, 2018
November 2nd, 2018

Chapters

Summary

This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

Transcript

Tags

Pre-assembled Plastic Microfluidic Chips Compartmentalizing Neurons Neuroscience Subcellular Manipulation Culture Devices Axon Development Synapse Remodeling Alzheimer's Disease Primary Rat Neurons Reproducible Method Silicon-based Devices High Resolution Microscopy Human Stem Cells Multicompartment Chip
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter