Adenoviral Gene Transfer
Adenoviral Gene Transfer - Related Videos
16:59
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
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Cited by 5 •
2012
We describe hepatic neo-islet formation in STZ (streptozotocin)-induced diabetic mice by gene transfer of Neurogenin3 (Ngn3) and Betacellulin (Btc) using helper-dependent adenoviral vector (HDAd) and the reversal of hyperglycemia. Our method takes advantages of helper-dependent adenoviral vectors with their highly efficient in vivo transduction and the long lasting gene expression.
12:30
Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Cited by 12 •
2014
These studies report on reversible attachment of adenoviral gene vectors to coatless metal surfaces of stents and model mesh disks. Sustained release of transduction-competent viral particles contingent upon hydrolysis of cross-linkers used for vector immobilization results in a durable site-specific transgene expression in vascular cells and in stented arteries.
03:20
Large-Scale Production of High-Capacity Adenoviral Vectors
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2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the large-scale amplification of high-capacity adenoviral vectors in suspension-adapted human embryonic kidney cells expressing Cre recombinase for use in gene delivery applications requiring high-titer, helper-free vector preparations.
15:33
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Cited by 7 •
2013
Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.
01:27
Horizontal Gene Transfer
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2025
Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
FAQs
Adenoviral gene transfer depends on a receptor-directed entry pathway. The viral capsid first recognizes receptors on the target-cell surface, enabling endocytosis. The particle must then escape the endosome so it can transport its DNA to the nucleus. These linked stages determine whether the genetic material reaches the cellular compartment where expression can occur.
After reaching the nucleus, the delivered DNA usually remains episomal, meaning it stays separate from the host chromosomes instead of integrating into the genome. Expression is consequently transient rather than permanently maintained. This characteristic is valuable when researchers need strong gene activity for a limited period or want to avoid sustained genomic integration.
Adenoviral vectors can infect dividing and nondividing cells, which broadens the biological systems available for study. Their ability to accommodate diverse genetic payloads further supports different goals, such as examining gene function or producing therapeutic proteins. Together, these properties make the approach applicable across varied cell contexts rather than one specialized cell type.
A basic workflow begins with a modified adenovirus carrying the selected genetic material and target cells prepared for exposure to the vector. The capsid binds cell-surface receptors, enters through endocytosis, escapes the endosome, and delivers DNA to the nucleus. Investigators then examine gene expression or protein production as the experimental outcome.
Adenoviral gene transfer supports work in molecular biology, vaccine development, cancer research, and investigations of gene-based therapies. Molecular biology studies can use it to examine gene function, while other projects may evaluate delivered genetic material in vaccine, cancer, or therapeutic contexts. The selected application depends on the research question and the needed expression pattern.
The principal outcome is gene expression from DNA delivered to the nucleus, sometimes including production of a desired therapeutic protein. Because adenoviral vectors provide strong but temporary expression, they suit experiments requiring a measurable response without permanent genomic integration. Results should therefore be understood as time-limited expression rather than sustained activity from an integrated gene.