Embryo Microinjection Protocol
Embryo Microinjection Protocol - Related Videos
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
05:51
Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo
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2023
This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.
03:10
Microinjection of Bacteria into the Caudal Vein of Zebrafish Embryos
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2026
Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.
03:58
Embryo Microinjection: A Technique to Deliver a Compound into the Zebrafish Yolk
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2023
This video describes a technique of Embryo microinjection to deliver compounds into Zebrafish yolk sac.
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
FAQs
Targeting the cytoplasm or pronucleus identifies where the introduced material first acts within the embryo. This choice makes compartment selection a key experimental variable when researchers study molecular changes during early development. Selecting the target requires coordinating pipette position, injection volume, and embryo stage so that delivery is controlled while cellular damage remains minimal and results are more reproducible.
The most important controllable variables are embryo stage, injection volume, pipette position, and handling conditions. Together, they influence whether the embryo tolerates the intervention and whether experiments can be reproduced consistently. Matching the procedure to the developmental stage and controlling the delivered volume helps researchers balance successful material delivery with minimized cellular damage when comparing manipulated embryos.
The delivered payload may be genetic material, proteins, or other substances, allowing the same platform to address distinct biological questions. Genetic material supports applications such as transgenesis and gene editing, while the broader approach can also support lineage tracing and developmental studies. Researchers therefore select the substance according to the molecular change or developmental process they want to investigate.
A basic workflow begins by immobilizing the early embryo and viewing it under a microscope. A fine glass micropipette is then positioned in the selected compartment, such as the cytoplasm or pronucleus, and the material is delivered while avoiding unnecessary cellular injury. Consistent control of positioning, volume, and handling conditions is central to obtaining embryos that survive the manipulation.
Successful work depends on coordinated use of microscopic guidance and a fine glass micropipette, together with handling conditions suited to the embryo. The microscope provides positional control, while the pipette enables delivery into a chosen compartment. Careful embryo handling during immobilization and injection is important because cellular damage can reduce survival and make experimental results less reproducible.
In biology, embryo microinjection connects controlled molecular manipulation with developmental research. It is used in developmental biology and reproductive research, and it also supports biotechnology. Resulting embryos can carry defined molecular changes for transgenesis, gene-editing studies, lineage tracing, or investigations of embryonic development. These applications help researchers relate an early intervention to developmental outcomes.