Antisense Oligos
Antisense Oligos - Related Videos
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
07:24
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos
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Cited by 17 •
2018
This manuscript describes the synthesis of a single-wall carbon nanotube (SWCNT)-conjugated MALAT1 antisense gapmer DNA oligonucleotide (SWCNT-anti-MALAT1), which demonstrates the reliable delivery of the SWCNT and the potent therapeutic effect of anti-MALAT1 in vitro and in vivo. Methods used for synthesis, modification, conjugation, and injection of SWCNT-anti-MALAT1 are described.
11:44
Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
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Cited by 6 •
2018
A protocol is described for the manual synthesis of oligo-peptoids followed by sequence analysis by mass spectrometry.
08:43
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
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Cited by 35 •
2010
A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.
10:30
Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy
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Cited by 23 •
2016
Exon skipping is currently a most promising therapeutic option for Duchenne muscular dystrophy (DMD). To expand the applicability for DMD patients and to optimize the stability/function of the resulting truncated dystrophin proteins, a multi-exon skipping approach using cocktail antisense oligonucleotides was developed and we demonstrated systemic dystrophin rescue in a dog model.
FAQs
The outcome depends on where the oligo binds and which RNA event is affected. Binding can support RNase H-mediated destruction of the complementary messenger RNA, whereas binding at a relevant processing site can block splicing or another RNA-processing event. Thus, sequence choice links the selected RNA region to either transcript reduction or a change in how its information is handled.
RNase H recruitment directs degradation of the recognized RNA, reducing the transcript itself. By contrast, translation blockade acts at the stage where RNA information would otherwise be used to produce protein. Distinguishing these outcomes helps researchers interpret whether an experiment primarily changes RNA abundance or protein production, an important distinction when studying gene regulation.
They can be designed to bind an RNA region involved in splicing and block that processing event. This approach does not merely lower the amount of a transcript; it aims to modify how the RNA is processed, making it useful for investigating abnormal splicing and for developing strategies that correct disease-associated RNA-processing patterns.
By directing regulation toward a selected messenger RNA, researchers can test how that transcript contributes to gene function, RNA biology, or a disease mechanism. The resulting ability to reduce, restore, or modify protein production makes antisense experiments useful for connecting a defined RNA target with a biological outcome.
They are relevant when a disease mechanism involves abnormal RNA processing or a disease-associated transcript. Researchers can use them to silence such transcripts, correct abnormal splicing, or explore how changing a selected RNA affects protein production. These applications connect molecular studies of RNA regulation with therapeutic development while also supporting investigation of disease biology.
By observing whether a targeted intervention reduces, restores, or modifies protein production, researchers can evaluate the functional contribution of the associated RNA. They can also examine whether the effect reflects transcript degradation, translation blocking, or altered splicing. These outcomes provide experimental context for studying gene function, RNA-processing mechanisms, disease-associated transcripts, and potential therapeutic strategies.