Apha-3 Gene

The Apha-3 gene is a segment of DNA that contains information used to produce a functional biological product, such as a protein or regulatory RNA, making it a potential contributor to cellular structure, signaling, or physiology. Its activity depends on gene regulation, in which transcription factors and other control elements influence transcription into messenger RNA, followed by translation when the gene encodes a protein. Studying the Apha-3 gene through sequencing, expression analysis, and genetic comparison can help researchers identify its role, examine variation among organisms or individuals, and connect altered activity with biological traits or disease-related processes.

Apha-3 Gene - Related Videos

Research

JoVE EoE - Neuropathology

Immunostaining of Neurons Treated with Alpha-Synuclein Aggregates

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2025

This video demonstrates the immunostaining of neurons treated with alpha-synuclein (αS) aggregates. The neurons are first treated with αS aggregates. They are then fixed, permeabilized, and treated with a blocking solution. Primary antibodies are added, followed by a fluorescently labeled secondary antibodies and a nuclear stain. The sample is then mounted and observed under a microscope.

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

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2009

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 3 describes the process of fluorescently labeling the amplified RNA from both host and viral samples by amino allyl coupling of dyes. Part 3 of 3.

Detection of Phosphorylated Alpha-Synuclein Using Western Blotting

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2025

Add a loading buffer containing a detergent, a reducing agent, and a tracking dye.

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

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Cited by 12 •

2016

This manuscript describes how viral vector-mediated local gene delivery provides an attractive way to express transgenes in the central nervous system. The protocol outlines all crucial steps to perform a viral vector injection in the substantia nigra of the rat to develop a viral vector-based animal model for Parkinson's disease.

Induction of Neuroinflammation in Mice via Injection of Alpha-Synuclein Fibrils

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2025

This video demonstrates the injection of alpha-synuclein fibrils into a mouse to induce neuroinflammation. The fibrils are injected intraperitoneally into a transgenic mouse expressing mutated alpha-synuclein proteins. Upon reaching the central nervous system, the fibrils enter neurons and induce aggregation of the mutated proteins. The aggregates spread to neighboring neurons, microglia, and astrocytes. Recognition of the aggregates triggers microglia to induce neuroinflammation, damaging the...

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