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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression
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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

Article DOI: 10.3791/1170 • 07:36 min • April 13th, 2009
April 13th, 2009 •
1, 1, 1
1Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

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Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 3 describes the process of fluorescently labeling the amplified RNA from both host and viral samples by amino allyl coupling of dyes. Part 3 of 3.

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Vaccinia Virus Infection Temporal Analysis Virus Gene Expression Poxviridae Family Orthopox Genus Variola Small Pox Monkeypox Vaccinia (VAC) Virus Genome Early Genes Intermediate Genes Late Genes Viral RNA Polymerases Host Cellular Environment Modulation Abundance Analysis HeLa Cells Time Course Infections RNA Isolation RNA Amplification Microarray Analysis Gene Expression
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