Brdu Staining
Brdu Staining - Related Videos
03:24
BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
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2023
This video describes the protocol for BrdU immunofluorescence staining. The technique allows the identification of cells in different phases of cell cycle. Addition of DNA dyes and antibody labeling allows the detailed analysis of the fate of the S phase cells at later times.
09:14
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Cited by 4 •
2016
In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA.
12:46
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
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Cited by 6 •
2011
Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
03:12
May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
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2023
This video describes the technique of staining bone marrow cells using May- Grünwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential counting.
FAQs
Because BrdU incorporation occurs during the S phase, a detected signal identifies cells actively synthesizing DNA during the labeling period. This timing allows investigators to compare the extent of DNA replication between tissues, developmental stages, experimental conditions, or treatments. The result therefore reflects a snapshot of proliferative activity rather than simply the presence of cells within a tissue.
DNA denaturation occurs after fixation and makes the incorporated BrdU accessible for antibody detection. Without this step, the labeled DNA may not be sufficiently exposed to anti-BrdU antibodies. Its position in the workflow is therefore essential for converting BrdU incorporation into a detectable staining signal that can be used to identify DNA-synthesizing cells.
Anti-BrdU antibodies recognize the BrdU incorporated into newly formed DNA after the DNA has been denatured. Antibody-based detection then marks cells containing labeled DNA, allowing researchers to identify and quantify proliferating cells. This molecular recognition connects the replication event itself with the visual or measured readout used in a biological experiment.
By measuring the number of BrdU-labeled cells in different samples, researchers can assess changes in DNA synthesis and proliferative activity. Comparisons may involve tissues, developmental stages, growth conditions, drugs, or other biological treatments. An increase or decrease in labeled cells provides evidence that the tested condition is associated with a corresponding change in cell growth.
The workflow begins with allowing cells to incorporate BrdU during DNA synthesis. Samples are then fixed to preserve cellular material, and the DNA is denatured so the incorporated analog can be detected. Anti-BrdU antibodies are applied to identify labeled DNA, after which the staining pattern can be examined to quantify cells undergoing replication.
BrdU staining supports research in development, neurogenesis, cancer biology, and tissue regeneration. It is also useful for evaluating how drugs or other biological treatments affect proliferation. Across these settings, the method provides a way to track DNA replication and compare proliferative activity among tissues or experimental groups.