Cell Aggregation
Cell Aggregation - Related Videos
08:59
4D Imaging of Protein Aggregation in Live Cells
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Cited by 4 •
2013
Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.
09:14
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
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Cited by 10 •
2011
A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.
04:16
Cell Aggregation Assay to Detect Trans Interactions between Cell Adhesion Molecules
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2025
In this video, we demonstrate the technique to measure transcellular interactions between synaptic cell adhesion molecules through the HEK cell aggregation assay. In this assay, an equal number of HEK cells expressing a desired presynaptic cell adhesion molecule and a fluorescent protein are mixed with HEK cells expressing a post-synaptic cell adhesion molecule and another fluorescent protein. The interaction between the cell adhesion molecules forces the cells to aggregate, which can be...
04:12
Protein Aggregate Formation Assay: A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor
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2025
In this video, we demonstrate a cell-based protein aggregation assay using proteasome inhibitors, which block proteasome activity, preventing misfolded, mutant proteins, fused to a fluorescent label, from undergoing ubiquitin-dependent proteasomal degradation, leading to their accumulation within the cell cytoplasm. The protein aggregates are then visualized and quantified by fluorescence microscopy.
10:10
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Cited by 29 •
2009
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
FAQs
Cadherins are cell-cell adhesion molecules that help neighboring cells attach to one another. Their adhesive activity supports compaction as individual cells become more closely associated within a cluster. This attachment is one part of a larger system: extracellular matrix interactions, cell contractility, and culture conditions also influence whether the resulting aggregate remains cohesive and how large it becomes.
Extracellular matrix interactions and cell contractility influence how cells organize and maintain physical cohesion after cell-cell attachment occurs. These factors can affect both cluster size and stability rather than acting as secondary features with no structural role. Considering them together helps researchers interpret why aggregates formed under different biological or culture conditions may develop different architectures.
Culture conditions help determine whether cells compact into stable clusters and what size those clusters achieve. Their effects operate alongside cadherin-mediated adhesion, extracellular matrix interactions, and cellular contractility. Consequently, changing the culture environment can alter the physical properties of an aggregate and may influence how effectively it supports studies of communication, differentiation, or tissue organization.
Researchers use aggregates to create multicellular structures that can model aspects of tissue development in three dimensions. The shared microenvironment allows cellular interactions and communication to be examined in an organized context rather than only as isolated-cell behavior. These models support investigations of how tissue architecture and cell relationships relate to developmental processes.
Cell aggregates provide a setting for examining how cellular interactions affect differentiation, signaling, and disease behavior. Because cells share a three-dimensional microenvironment, researchers can study these outcomes in relation to aggregate organization and stability. This makes aggregates useful for connecting physical cell arrangement with biological responses relevant to both normal tissue processes and disease research.
Controlled aggregation is useful when researchers need multicellular structures for tissue engineering or regenerative medicine. Aggregates can also support the generation of spheroids and organoids for biological research. By managing how cells associate and compact, investigators create organized structures that can be studied directly or used as components in efforts to develop engineered tissues.