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JoVE Journal
Biology

This content is Open Access.

4D Imaging of Protein Aggregation in Live Cells
 

4D Imaging of Protein Aggregation in Live Cells

Article DOI: 10.3791/50083
April 5th, 2013

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Summary April 5th, 2013

Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.

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