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April 05, 2013
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Summary
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Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.
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Cite this Article
Spokoini, R., Shamir, M., Keness, A., Kaganovich, D. 4D Imaging of Protein Aggregation in Live Cells. J. Vis. Exp. (74), e50083, doi:10.3791/50083 (2013).
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