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4D Imaging of Protein Aggregation in Live Cells
4D Imaging of Protein Aggregation in Live Cells
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
4D Imaging of Protein Aggregation in Live Cells

4D Imaging of Protein Aggregation in Live Cells

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08:59 min

April 05, 2013

DOI:

08:59 min
April 05, 2013

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Summary

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Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.

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