Colony Picking
Colony Picking - Related Videos
07:28
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
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Cited by 12 •
2015
TALEN-mediated gene editing at the safe harbor AAVS1 locus enables high-efficiency transgene addition in human iPSCs. This protocol describes the procedures for preparing iPSCs for TALEN and donor vector delivery, transfecting iPSCs, and selecting and isolating iPSC clones to achieve targeted integration of a GFP gene to generate reporter lines.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
07:47
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
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Cited by 66 •
2014
ScanLag is a high-throughput method for measuring the delay in growth, namely lag time, as well as the growth rate of colonies for thousands of cells in parallel. Screening using ScanLag enables the discrimination between long lag-time and slow growth at the level of a single variant.
02:38
Isolating Induced Neural Progenitor Cell Colonies Derived From Adult Human Fibroblasts
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2025
This video demonstrates a technique for isolating induced neural progenitor cells (iNPCs) derived from adult human fibroblasts. It outlines the steps involved in isolating the iNPC colonies and culturing them in laminin-coated multi-well plate wells in the presence of suitable culture media that facilitate the continued proliferation and expansion of the iNPC population.
13:11
Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
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Cited by 17 •
2010
Video bioinformatics is the automated processing, analysis, understanding, and data mining of biological spatio-temporal data extracted from microscopic videos. The purpose of this article is to demonstrate a method for measuring human embryonic stem cell colony growth using a video bioinformatics method.
FAQs
A visible colony typically develops from one founding cell or a cell cluster, so transferring it can establish a culture with a relatively consistent biological origin. This supports isolation of individual microbial strains and makes later growth, identification, characterization, or phenotype comparisons more reproducible than sampling an undifferentiated mixed population.
Selection depends on choosing an appropriate visible colony and preventing unwanted cells from entering the transfer. Aseptic handling helps preserve the selected population rather than introducing contaminants or neighboring colonies. Together, careful selection and clean transfer improve the likelihood that the resulting culture represents the intended strain or genetic construct.
Manual picking uses a sterile tool to transfer a selected colony, whereas an automated picker performs the transfer through an instrument-based workflow. Both approaches serve the same biological purpose, but the source material identifies automation as an option for handling colony transfers. The chosen approach can support strain isolation, maintenance, or downstream screening.
The workflow begins with microbial growth on a solid agar medium until colonies become visible. A suitable colony is then selected and transferred with a sterile tool or automated picker into fresh liquid medium or onto another plate. Subsequent growth provides material for downstream analysis, maintenance, identification, or characterization.
In recombinant DNA work, colony picking helps separate individual colonies for evaluation of desired genetic constructs. Selected colonies can be transferred to fresh growth conditions, allowing each candidate population to be maintained and examined independently. This supports screening workflows in which researchers compare colonies for the presence of a target construct or associated phenotype.
Colony picking can generate cultures suitable for bacterial isolation, strain maintenance, and screening for desired phenotypes or genetic constructs. The resulting material may then support identification and characterization of selected populations. By linking each downstream sample to a chosen colony, the technique strengthens experimental reproducibility in microbiology and biotechnology studies.