Concanavalin A Adhesion
Concanavalin A Adhesion - Related Videos
04:02
Ultrasound-Guided Concanavalin A Injection Into Inferior Lacrimal Gland: A Technique to Inoculate Inferior Lacrimal Gland With Concanavalin A to Induce Dry Eye Disease in Rabbit Model
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2025
In this video, we demonstrate the inoculation of Concanavalin A, a lymphocyte mitogen, into the inferior lacrimal gland of the eye of a rabbit to induce dry eye disease. Ultrasound or sonogram images are used to guide the precise location of the injection site and also to confirm the success of inoculation.
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
13:28
Introducing Shear Stress in the Study of Bacterial Adhesion
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Cited by 9 •
2011
During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.
07:40
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Cited by 3 •
2016
This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.
06:44
Creation of Abdominal Adhesions in Mice
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Cited by 9 •
2016
Abdominal adhesions that form after surgery are a major cause of pain, infertility, and hospitalization and reoperation for small bowel obstruction. Our surgical procedure for creating abdominal adhesions in mice is a reliable tool to study the mechanisms underlying the formation of adhesions.
FAQs
These carbohydrate residues provide recognition sites on membrane glycoproteins and glycolipids. When Concanavalin A binds them, it can connect neighboring surface glycoconjugates rather than interacting with an isolated membrane component. This cross-linking changes how cellular surfaces interact, helping explain increased cell-cell or cell-substrate attachment in a biological assay.
Cross-linking brings carbohydrate-bearing molecules into a more connected surface arrangement. That organization can strengthen interactions between cells or between a cell and its substrate under suitable conditions. Consequently, the adhesion response reflects not only lectin binding, but also how membrane glycoconjugates are distributed and organized at the cellular surface.
The adhesion response depends on accessible mannose- and glucose-containing groups on surface glycoproteins and glycolipids. If cell-surface glycosylation changes, the available recognition sites and resulting attachment behavior may also change. Measuring adhesion therefore provides an indirect way to examine alterations in membrane carbohydrate composition or presentation.
Treatments that modify membrane structure or function may change how surface glycoconjugates are presented or how effectively they participate in cross-linking. Such changes can influence cell-cell interactions, cell-substrate attachment, or overall adhesion behavior. The assay is therefore useful for relating treatment-induced membrane changes to an observable cellular response.
The assay can be used to evaluate attachment behavior and changes in interactions between cells or between cells and a substrate. Interpreting the result alongside information about cell-surface glycosylation or membrane condition helps connect the observed adhesion response with changes in cellular surface organization and function.
This approach provides a model for investigating carbohydrate-mediated recognition, membrane organization, and adhesion mechanisms. It also supports studies of how altered glycosylation or membrane function affects cellular attachment. Because the response can involve either cell-cell or cell-substrate interactions, the method connects molecular surface features with broader cellular behavior.