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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

DOI:
10.3791/54203-v

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07:40 min

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June 29, 2016

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, , ,
1Department of Medicine,New York University School of Medicine, 2Department of Pathology,New York University School of Medicine

Chapters

  • 00:05Title
  • 00:48Chinese Hamster Ovary (CHO)-Intercellular Adhesion Molecule (ICAM) Cell Plating
  • 01:50T Cell Preparation
  • 02:46Input/Output Hosing and Syringe Pump Preparation
  • 03:59Flow Chamber Loading and Cell Stimulation
  • 05:53Results: SDF-1alpha Induced T Cell Adhesion to ICAM-1 Under Shear Stress
  • 06:51Conclusion

Summary

Automatic Translation

Automatic Translation

This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.

Tags

Keywords: AdhesionShear StressT LymphocyteAdhesion MoleculeIntegrinLFA1CHO-ICAMT CellsCFSEFlow ChamberLive Imaging

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