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JoVE Journal
Immunology and Infection

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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
 

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Article DOI: 10.3791/54203 07:40 min June 29th, 2016
June 29th, 2016

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Summary

This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.

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Assay Adhesion Shear Stress T Lymphocyte Adhesion Molecule Interactions Treatments Cellular Adhesion Immune Cell Signaling Integrin Activation Advantage Flow System LFA1 Activation Integrins Technical Setup CHO-ICAM Cells Trypsin EDTA Dissociated Cells Centrifuge Flow Chamber Culture Medium
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