Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

The overall goal of this assay is to determine the effects of treatments of interest on cellular adhesion under shear stress conditions. This method can help answer key questions in the immune cell signaling field, such as, what factors mediate integrin activation. The main advantage of this technique is that single adhesion molecule integrin pairs can be studied in an easily replicated flow system.

Though this method can provide insight into LFA1 activation, it can also be applied to the study of other integrins. Generally, individuals new to this method may struggle because the technical setup must be precisely executed. To harvest the CHO-ICAM cells, treat the culture with 0.5%trypsin EDTA for one minute at room temperature with gentle shaking.