Cork Cambium

Cork cambium, or phellogen, is a lateral meristem in woody plants that produces protective tissues during secondary growth. Its dividing cells generate cork, or phellem, toward the outside and phelloderm toward the inside, forming the periderm that replaces the epidermis as stems and roots expand. Cork cells accumulate suberin, a waxy substance that limits water loss and helps protect underlying tissues from pathogens, physical damage, and temperature fluctuations. Studying cork cambium helps explain bark formation, stem and root thickening, wound responses, and the structural adaptations that enable long-lived plants to survive in changing environments.

Cork Cambium - Related Videos

Research

JoVE Journal - Engineering

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

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Cited by 10 •

2013

We discussed the synthesis of individual graphitic nanocups using a series of techniques including chemical vapor deposition, acid oxidation and probe-tip sonication. By citrate reduction of HAuCl4, the graphitic nanocups were effectively corked with gold nanoparticles due to the chemically reactive edges of the cups.

Bioluminescent Bacterial Imaging In Vivo

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Cited by 9 •

2012

This article describes the administration of lux-tagged bacteria to mice and subsequent in vivo analysis using IVIS bioluminescence imaging.

Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery

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Cited by 4 •

2010

This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.

Research

JoVE Journal - Biology
Free Sample

Quantification of Cytokine-Induced Cell Death in Human Colonic Organoids Using Live Fluorescence Microscopy

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2024

This protocol describes a simple and cost-effective method to investigate and quantify cell death in human colonic organoids in response to cytotoxic perturbagens such as cytokines. The approach employs a fluorescent cell death dye (SYTOX Green Nucleic Acid Stain), live fluorescence microscopy, and open-source image analysis software to quantify single-organoid responses to cytotoxic stimuli.

Murine Bioluminescent Hepatic Tumour Model

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Cited by 11 •

2010

This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.

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