Murine Bioluminescent Hepatic Tumour Model

Journal

Medicine

JoVE Journal
Medicine
Author Produced

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JoVE Journal Medicine

Murine Bioluminescent Hepatic Tumour Model

DOI:

10.3791/1977-v

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05:23 min

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July 17, 2010

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Simon Rajendran, Slawomir Salwa, Xuefeng Gao, Sabin Tabirca, Deirdre O’Hanlon, Gerald C. O’Sullivan, Mark Tangney

¹Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory,University College Cork, ²Department of Computer Science,University College Cork, ³South Infirmary Victoria University Hospital

Chapters

00:00Title
00:05Introduction
01:24Materials
02:12Preparation of the Mouse
02:49Abdominal Incision
02:58Exposureand Division of Spleen
03:17Injection of Cells into One Hemi-Spleen
03:50Excision of Injection Hemi-Spleen
03:55Abdominal Closure
04:13Whole Body Imaging

Summary

Automatic Translation

This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.

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Chapters

Summary

Automatic Translation

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