Crosslinking Agents
Crosslinking Agents - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
11:27
Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
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Cited by 5 •
2016
This protocol describes the preparation and characterization of a dendrimeric magnetic resonance imaging (MRI) contrast agent that carries cyclen-based macrocyclic chelates coordinating paramagnetic gadolinium ions. In a series of MRI experiments in vitro, this agent produced an amplified MRI signal when compared to the commercially available monomeric analogue.
10:19
Reducing Agents
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2023
Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA Controlling the reactivity and selectivity during the synthesis of a molecule is very important criteria for chemists. This has led to the development of many reagents that allow chemists to pick and choose reagents suitable for a given task. Quite often, a balance between reactivity and selectivity needs to be achieved. This experiment will use IR spectroscopy to monitor the reaction and to...
FAQs
Covalent crosslinks connect neighboring molecular components through direct chemical bonds, whereas reversible crosslinks rely on interactions that can be undone. This distinction matters when selecting how a biological material should be stabilized. The resulting network can differ in strength, stability, and other physical properties depending on the type of connection formed.
Crosslinking efficiency depends on agent concentration, pH, temperature, and exposure time. These variables should therefore be selected and maintained in relation to the biological material and intended outcome, rather than treated as interchangeable settings. Controlling them helps produce a network with the desired level of strength, stability, or altered physical behavior.
Functional groups provide the molecular sites through which neighboring proteins, nucleic acids, polysaccharides, or synthetic polymers can become connected. Their presence determines which components can participate in network formation and helps explain why different biological materials respond differently. Considering these groups is important when stabilizing a material or capturing molecular associations.
Their role in microscopy is to preserve cellular and tissue architecture so structural organization remains available for examination. Stabilizing the sample helps maintain the arrangement of biological components during preparation and analysis. This application emphasizes preservation of spatial structure, allowing microscopy to investigate architecture rather than observing a material whose organization has been lost.
Crosslinking agents can stabilize biomaterials and hydrogels, while controlled network formation supports the creation of scaffolds for tissue engineering. These uses connect molecular-level interactions with material-level properties, including strength and stability. The approach is therefore relevant when researchers need a biological material or hydrogel with a defined structural framework for further application.
They can capture interactions that might otherwise dissociate during analysis by connecting the associated molecular components. This preserves evidence of relationships among biological molecules long enough for molecular studies to examine them. The strategy is especially useful when the interaction itself is difficult to maintain under the conditions required for subsequent analysis.