Fibered Confocal Microscopy
Fibered Confocal Microscopy - Related Videos
06:07
Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network
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2025
This method visualizes the native structure of collagen fibers in the vitreous body using confocal reflectance microscopy.
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
04:07
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
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2025
Source: Marinello, M., et al. Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy. J. Vis. Exp. (2021)This video demonstrates the preparation, immunostaining, and imaging of neuromuscular junctions in rat muscle tissue using confocal and super-resolution microscopy to visualize and analyze presynaptic and postsynaptic structures.
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
FAQs
The pinhole suppresses light originating outside the selected focal plane. By rejecting out-of-focus emitted or reflected signals before image collection, it helps preserve optical sectioning and improves the distinction between structures located at different depths. This capability is important when examining tissue organization or cellular morphology within complex biological samples.
Excitation light travels through the fiber toward the biological sample, while emitted or reflected light returns through the same fiber toward the confocal detection path. This arrangement allows illumination and signal collection through a compact probe, supporting imaging at locations that a conventional microscope objective may not readily reach.
The fiber probe provides access to regions that are difficult to examine with conventional microscope objectives. Its small, reachable imaging interface supports localized observations inside biological tissues and can be used in living systems. Consequently, investigators can relate microscopic structural features to processes occurring in biologically relevant locations.
A typical examination positions the fiber probe at the tissue region of interest, sends excitation light through the fiber, and collects emitted or reflected light returning from the sample. The confocal arrangement then suppresses out-of-focus signals so that thin focal planes can be examined and compared across the biological specimen.
The method can reveal cellular morphology, tissue organization, and changes associated with disease. Because it can support real-time imaging in living systems, observations may connect structural patterns with dynamic biological processes rather than limiting analysis to static samples. This combination makes the technique useful for studying how tissue appearance changes during disease-related events.
It is especially useful when researchers need microscopic information from tissue locations that conventional objectives cannot readily access or when they want observations from living systems over time. The resulting images can link microscopic structure with dynamic processes and may help guide investigations related to diagnostics and therapeutics.