Fluorescent Reporter
Fluorescent Reporter - Related Videos
03:04
Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa
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2025
Source: Rugjee, K. N. et. al., Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa. J. Vis. Exp. (2016)This video demonstrates the generation of a cyclic-di-GMP-responsive reporter strain in Pseudomonas aeruginosa using electroporation. Successful transformation is confirmed through antibiotic selection and fluorescence-based...
02:45
Assessment of Viral Fusion Inhibitors Using a Cre-Inducible Fluorescent Reporter
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2026
Source: Esposito, A. M.,et al. High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (2018)This video demonstrates a co-culture fluorescent assay to monitor cell-to-cell viral transmission. The assay uses a red-to-green reporter cassette to visualize viral membrane fusion and evaluate the efficacy of test compounds in blocking viral entry.
03:49
A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
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2025
This video demonstrates a method to track viral infections in mouse models using a recombinant luciferase and fluorescence protein-expressing bi-reporter Influenza A virus. This method enables both in vivo and ex vivo studies, enabling researchers to gain insights into viral dynamics and the effects of interventions.
05:26
Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection
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2025
This video demonstrates the reporter enzyme fluorescence (REF) imaging for in vivo detection of Mycobacterium tuberculosis infection. The infected mice are intraperitoneally injected with a substrate for REF imaging. Upon being hydrolyzed by bacterial β-lactamase enzyme, the substrate emits fluorescence, the detection of which aids evaluation of infection.
04:13
Imaging Neurotransmitter Release Using Cell-Based Neurotransmitter Fluorescent Engineered Reporters
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2025
Source: Lacin, E., et al. Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo. J. Vis. Exp. (2016)This video demonstrates a technique for imaging neurotransmitter release in vivo using cell-based neurotransmitter fluorescent engineered reporters (CNiFERs), specialized reporter cells pre-injected into the mouse cortex. Positioned under a two-photon microscope, the mouse allows visualization of...
FAQs
The fluorophore absorbs excitation light and then emits light at a longer wavelength, creating a signal that microscopy can detect. When the reporter is linked to a biological process, changes in the emitted signal can be observed in cells or tissues. This optical conversion allows researchers to follow activity in living systems rather than relying only on endpoint measurements.
A reporter positioned under a chosen promoter primarily provides a readout of gene expression associated with that regulatory sequence. A reporter fused to a protein of interest instead follows that protein’s localization within the cell or tissue. The two designs therefore answer different questions: one emphasizes expression patterns, while the other reveals where a protein is present.
The separation between excitation and emission wavelengths enables the emitted signal to be distinguished from the light used to stimulate the fluorophore. That distinction makes fluorescence detectable through microscopy and supports visualization in cells and tissues. As a result, researchers can observe reporter-associated patterns while preserving the connection between the optical signal and the underlying biological process.
Because fluorescent reporters can be monitored in living cells and tissues, imaging can follow biological processes as they change rather than examining only a single fixed state. Quantitative imaging adds a way to evaluate differences in the detected signal across observations. This is useful for studying dynamic cellular mechanisms, signaling behavior, and responses to experimental treatments.
A typical workflow begins by choosing whether the experiment requires a promoter-linked reporter for gene expression or a fusion with a protein of interest for localization. The construct is then used in the relevant biological system, and microscopy detects the resulting fluorescence. Researchers can examine the observed pattern or quantify changes to address the experimental question.
This approach is useful when the goal is to visualize where or when expression associated with a chosen promoter occurs. Reporter fluorescence can be examined in living cells or tissues, allowing expression patterns to be related to developmental or disease-related processes. The method is particularly valuable when researchers need spatial information together with observations over time.
In cell signaling studies, reporter signals can help monitor activity as it changes within living cells. In developmental research, imaging can follow biological processes across tissues over time, while disease studies can visualize disease-related changes. Researchers can also use quantitative imaging to evaluate how experimental treatments affect these processes, connecting measurable fluorescence with biological outcomes.