Human T-cell Assays
Human T-cell Assays - Related Videos
03:28
Live-cell Phagocytosis Assay of Microglia-Like Cells Using Human Synaptosomes
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2025
Source: Funes, S., et. al., Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes. J. Vis. Exp. (2022)This video demonstrates an assay to study the phagocytic capacity of microglia-like cells using pH-sensitive fluorescent dye-labeled synaptosomes.
05:30
Clonogenic Assay: Adherent Cells
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Cited by 228 •
2011
The applicability of the clonogenic assay for evaluating reproductive viability has been established for more than 50 years. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cells.
02:59
Luciferase Assay for Measuring γ-Secretase Activity in Human Cells
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2025
This video demonstrates the procedure of measuring γ-secretase activity in genetically modified human cells using a tetracycline-inducible system. The process involves inducing APP gene expression with tetracycline, generating amyloid-β peptides, and activating luciferase production. The bioluminescence resulting from the luciferase-substrate reaction is then measured, reflecting γ-secretase activity.
11:30
Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
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Cited by 43 •
2010
The colony forming cell (CFC) assay is an in vitro assay in which hematopoietic progenitors form colonies in a semi-solid medium. A combination of colony morphology, cell morphology, and flow cytometry are used to assess the ability of the progenitors to proliferate and differentiate along the different hematopoietic lineages.
12:00
Titration of Human Coronaviruses Using an Immunoperoxidase Assay
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Cited by 14 •
2008
In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.
FAQs
The selected stimulus determines whether the assay examines recognition of a defined antigen or a broader response to activating signals. This distinction helps researchers interpret whether observed T-cell activity reflects antigen-specific behavior or general cellular activation. Matching the stimulation approach to the biological question is therefore important when comparing responses across experimental conditions.
Each readout describes a different aspect of T-cell activity. Proliferation indicates cellular expansion, cytokine release reflects secretory function, cytotoxicity measures killing activity, and surface-marker expression identifies changes in the cell phenotype. Flow cytometry can assess surface markers, whereas immunoassays can quantify released cytokines, allowing complementary views of the same response.
Donor samples, culture conditions, and stimulation protocols can all influence the activity measured in an assay. If these factors vary between experiments, differences in proliferation, cytokine release, cytotoxicity, or marker expression may become difficult to interpret. Careful control of these variables improves reproducibility and supports more reliable comparisons across conditions.
The most useful readout depends on the function being investigated. Researchers can select proliferation to examine expansion, cytokine release to assess secretory responses, cytotoxicity to study killing activity, or surface-marker expression to characterize cellular changes. Using a readout that matches the experimental objective makes the resulting measurements more biologically informative.
These assays help researchers evaluate how T cells respond to vaccine-related stimulation or therapeutic intervention. Measurements of proliferation, cytokine release, cytotoxicity, and surface-marker expression can reveal changes in cellular activity across conditions. Such comparisons support characterization of immune responses and assessment of therapeutic responses in experimental studies.
In infection and cancer studies, these assays can characterize antigen-specific responses and assess functional activity such as cytokine release or cytotoxicity. In immune-disorder research, comparing cellular measurements across conditions can help identify altered responses. Their relevance to biology comes from linking T-cell activity with adaptive immune function in different disease or treatment contexts.