Clonogenic Assay: Adherent Cells

Haloom Rafehi; Christian Orlowski; George T. Georgiadis; Katherine Ververis; Assam El-Osta; Tom C. Karagiannis

doi:10.3791/2573

Journal

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Biology

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Clonogenic Assay: Adherent Cells

JoVE Journal
Biology
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JoVE Journal Biology

Clonogenic Assay: Adherent Cells

47,879 Views

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05:30 min

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March 13, 2011

DOI:

10.3791/2573-v

DOI:

10.3791/2573-v

•

05:30 min

March 13, 2011

•

35 Views

Haloom Rafehi^1,2, Christian Orlowski^1,2,3, George T. Georgiadis¹, Katherine Ververis^1,4, Assam El-Osta^2,3, Tom C. Karagiannis^1,2

¹Epigenomic Medicine, BakerIDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, ²Department of Pathology,The University of Melbourne, ³Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, ⁴Department of Anatomy and Cellular Biology,The University of Melbourne

Summary

The applicability of the clonogenic assay for evaluating reproductive viability has been established for more than 50 years. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cells.

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Clonogenic Assay: Adherent Cells

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Summary

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