Hypoxia Induction
Hypoxia Induction - Related Videos
08:01
Induction of Hypoxia in Living Frog and Zebrafish Embryos
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Cited by 5 •
2017
We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
02:54
Hypoxia-Induced Climbing Behavior of Drosophila melanogaster
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2026
Source:Siddarth Gunnala1, Nafisa M. Jadavji2,3,41Department of Biomedical Sciences, Midwestern University, Glendale, AZ, USA2Division of Integrative and Molecular Physiology, Department of Biomedical Sciences, Southern University of Illinois, Carbondale, IL, USA3Department of Child Health, College of Medicine Phoenix, University of Arizona, Phoenix, AZ, USA4Department of Neuroscience, Carleton University, Ottawa, ON, CanadaThis video demonstrates hypoxia-induced climbing behavior in Drosophila...
07:10
Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model
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Cited by 7 •
2020
This protocol describes the induction of pulmonary hypertension (PH) in mice based on the exposure to hypoxia and the injection of a VEGF receptor antagonist. The animals develop PH and right ventricular (RV) hypertrophy 3 weeks after the initiation of the protocol. The functional and morphometrical characterization of the model is also presented.
06:01
A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia
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Cited by 8 •
2015
Thromboembolic stroke models are vital tools for optimizing the recanalization therapy. Here we report a murine thrombotic stroke model based on transient cerebral hypoxic-ischemic (tHI) insult, which triggers thrombosis and infarction, and responds favorably to tissue plasminogen activator (tPA)-mediated fibrinolysis in a therapeutic window similar to those in stroke patients.
FAQs
Reduced oxygen availability inhibits oxygen-dependent prolyl hydroxylation of hypoxia-inducible factors, or HIF proteins. When this modification is inhibited, HIF proteins accumulate and enter the nucleus, where they activate genes associated with metabolism, angiogenesis, and survival. This oxygen-sensing mechanism connects the environmental condition to measurable changes in cellular behavior and gene activity.
HIF accumulation provides a central link between oxygen limitation and adaptive gene regulation. After entering the nucleus, HIF proteins activate genes involved in cellular metabolism, blood-vessel formation, and survival. Studying these downstream responses helps researchers examine how cells, tissues, or organisms adjust their biology when oxygen availability is reduced.
Hypoxia induction can rely on either controlled oxygen reduction or chemical agents that mimic oxygen deprivation. Oxygen-controlled systems alter the availability of oxygen directly, whereas chemical approaches reproduce aspects of the oxygen-limitation response without necessarily changing the surrounding oxygen level. The choice allows researchers to investigate oxygen-sensing pathways through different experimental strategies.
A study begins by selecting the biological system, such as cultured cells, tissue, or an organism, and then choosing an induction strategy. Researchers can control oxygen levels in the experimental system or apply a chemical agent that mimics oxygen deprivation. They then examine responses linked to HIF activity, including changes related to metabolism, angiogenesis, or survival.
This approach is useful when researchers need to examine how oxygen limitation affects biological systems. Applications include studying developmental adaptation, tumor biology, ischemic injury, inflammation, and cellular energy regulation. Because the method activates oxygen-sensing responses, it also supports investigations into how altered oxygen availability contributes to disease-related or adaptive processes.
By creating experimental conditions that activate oxygen-sensing responses, hypoxia induction provides a setting for evaluating therapies directed at those pathways. Researchers can examine how treatment affects processes associated with HIF activity, including metabolism, angiogenesis, and survival. This makes the approach relevant to investigations of tumor biology, ischemic injury, inflammation, and related biological responses.