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Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model
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Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model

Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model

DOI:
10.3791/59252-v

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07:10 min

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June 03, 2020

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, , ,
1Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, 2Phospholamban Foundation

Chapters

  • 00:04Introduction
  • 00:41Pulmonary Hypertension (PH) Induction
  • 02:12Intubation
  • 04:23Right Ventricular (RV) Pressure Measurement
  • 05:39Results: Representative Mouse Pulmonary Hypertension Characterization
  • 06:36Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the induction of pulmonary hypertension (PH) in mice based on the exposure to hypoxia and the injection of a VEGF receptor antagonist. The animals develop PH and right ventricular (RV) hypertrophy 3 weeks after the initiation of the protocol. The functional and morphometrical characterization of the model is also presented.

Tags

Keywords: Pulmonary HypertensionHypoxia/SU5416 ModelRight VentricleInduction ProcedureSU5416 InjectionHypoxia ExposurePressure MeasurementPressure Volume CatheterAnesthesiaTemperature Monitoring

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