Lord Howe Woodhen

The Lord Howe Woodhen is a flightless rail endemic to Lord Howe Island, Australia, and a notable example of island evolution and species conservation. Geographic isolation and limited exposure to terrestrial predators favored reduced flight ability, while strong legs and ground-foraging behavior supported movement through the island’s forest and palm habitats. Introduced predators, habitat pressures, and human activity caused severe population decline, making the species a focus of recovery biology. Captive breeding, predator management, habitat protection, and reintroduction programs have demonstrated how targeted conservation can restore highly threatened island birds and inform broader strategies for preserving endemic biodiversity.

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JoVE Journal - Immunology and Infection

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A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.

Chromosome Preparation From Cultured Cells

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Cited by 146 •

2014

Chromosomes can be isolated from live cells such as lymphocytes or skin fibroblasts, and from organisms including humans or mice. These chromosome preparations can be further utilized for routine G-banding and molecular cytogenetic procedures such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and spectral karyotyping (SKY).

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2016

Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.

Research

JoVE Journal - Medicine
Free Sample

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2013

Effective pulmonary vein isolation utilizing a cryoballoon depends on complete pulmonary vein occlusion. The point of occlusion can be effectively predicted by direct analysis of pulmonary vein pressure waveform analysis during balloon inflation using a simple and reproducible technique.

Zebrafish Model of Neuroblastoma Metastasis

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Cited by 4 •

2021

This paper introduces the method of developing, characterizing, and tracking in real-time the tumor metastasis in zebrafish model of neuroblastoma, specifically in the transgenic zebrafish line with overexpression of MYCN and LMO1, which develops metastasis spontaneously.

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