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Zebrafish Model of Neuroblastoma Metastasis
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JoVE Journal Cancer Research
Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

DOI:
10.3791/62416-v

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05:20 min

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March 14, 2021

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, , , ,
1Department of Biochemistry and Molecular Biology,Mayo Clinic College of Medicine, Mayo Clinic Cancer Center

Chapters

  • 00:04Introduction
  • 00:57Outcross of LMO1 and MYCN Transgenic Lines to Create Metastatic Model
  • 02:11Visualizing Tumor Burden in Transgenic Zebrafish Lines
  • 03:17Results: Transgenic Zebrafish Model Overexpressing MYCN and LMO1 for Neuroblastoma Metastasis
  • 04:50Conclusion

Summary

Automatic Translation

Automatic Translation

This paper introduces the method of developing, characterizing, and tracking in real-time the tumor metastasis in zebrafish model of neuroblastoma, specifically in the transgenic zebrafish line with overexpression of MYCN and LMO1, which develops metastasis spontaneously.

Tags

Keywords: ZebrafishNeuroblastomaMetastasisIn Vivo ImagingTumor DisseminationMYCNLMO1TransgenicEGFPFluorescence MicroscopeTumor ScreeningTumor-bearing FishTumor Cell Migration

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