80,710 Views
•
07:42 min
•
January 28, 2014
DOI:
Summary
Automatically generated
Chromosomes can be isolated from live cells such as lymphocytes or skin fibroblasts, and from organisms including humans or mice. These chromosome preparations can be further utilized for routine G-banding and molecular cytogenetic procedures such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and spectral karyotyping (SKY).
Related Videos
05:47
Counting and Determining the Viability of Cultured Cells
Related Videos
21906 Views
08:20
Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
Related Videos
73208 Views
06:37
Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
Related Videos
31015 Views
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Related Videos
21076 Views
16:43
Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Related Videos
12900 Views
11:03
Massively Parallel Reporter Assays in Cultured Mammalian Cells
Related Videos
21391 Views
09:27
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Related Videos
39278 Views
09:30
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
Related Videos
10675 Views
09:08
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Related Videos
13139 Views
06:38
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
Related Videos
11651 Views
Read Article
Cite this Article
Howe, B., Umrigar, A., Tsien, F. Chromosome Preparation From Cultured Cells. J. Vis. Exp. (83), e50203, doi:10.3791/50203 (2014).
Copy