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Chromosome Preparation From Cultured Cells
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Chromosome Preparation From Cultured Cells

Chromosome Preparation From Cultured Cells

DOI:
10.3791/50203-v

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07:42 min

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January 28, 2014

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, ,
1Department of Genetics,Louisiana State University Health Science Center

Chapters

  • 00:05Title
  • 01:41Chromosome Harvesting of Adherent Cells
  • 03:52Slide preparation and Solid Staining
  • 05:35Banding Using Trypsin and Giemsa (GTG)
  • 06:44Results: Chromosome Spreads
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

Chromosomes can be isolated from live cells such as lymphocytes or skin fibroblasts, and from organisms including humans or mice. These chromosome preparations can be further utilized for routine G-banding and molecular cytogenetic procedures such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and spectral karyotyping (SKY).

Tags

Keywords: Chromosome PreparationChromosome AnalysisCytogenetic AnalysisChromosome AbnormalitiesG-bandingFISHCGHSKYCell CultureMetaphaseMitosisColcemidHypotonic SolutionCarnoy’s FixativeGene TherapyStem Cell Research

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