Lymphoid Depletion
Lymphoid Depletion - Related Videos
06:17
Depletion of Specific Cell Populations by Complement Depletion
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Cited by 15 •
2010
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
08:50
Depletion and Reconstitution of Macrophages in Mice
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Cited by 118 •
2012
Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.
04:39
In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors
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2025
This video demonstrates an in vitro assay to generate plasmacytoid dendritic cells (pDCs) from common lymphoid progenitor cells (CLPs). Upon co-culturing purified CLPs with γ-irradiated feeder cells in the presence of Fms-like tyrosine kinase-3 ligand (FL), the CLPs differentiate into non-adherent pDCs, the presence of which is confirmed using flow cytometry.
01:18
Lymphoid Cells and Tissues
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2024
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes. Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
01:15
Secondary Lymphoid Organs
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2024
Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection. The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
FAQs
The outcome depends on how lymphoid cells are affected. Cytotoxic drugs may damage these cells or inhibit their proliferation, irradiation can reduce them through damaging exposure, and targeted antibodies can promote selective elimination. These distinct mechanisms can produce different degrees and patterns of depletion, which matters when linking an experimental intervention to immune-system changes.
Selective elimination is useful when a study needs to focus on particular lymphoid populations rather than reduce lymphoid cells broadly. By contrast, cytotoxic drugs or irradiation may create wider changes in the lymphoid compartment. This distinction helps researchers decide whether an observed immune effect reflects loss of a targeted population or a more generalized reduction.
Depletion is not a static endpoint because lymphoid tissues may recover and repopulate after the initial reduction. Measuring both the extent of cell loss and its timing allows investigators to distinguish immediate effects from later changes. This temporal information is essential when interpreting immune defense, inflammation, tolerance, or treatment responses during recovery.
A study should establish when depletion occurs, how extensive it becomes, and whether lymphoid tissues later repopulate. Investigators can relate changes in the experimental sample or organism to the depletion timeline, then evaluate how immune activity changes. This framework supports clearer interpretation when the intervention affects several aspects of lymphoid biology.
Researchers apply lymphoid depletion to examine immune-cell function and to study how lymphoid tissues recover after cell loss. It can also help prepare experimental models for transplantation or broader immunological investigation. In each setting, the value of the model depends on connecting the induced depletion with subsequent changes in immune-system activity.
Changes after depletion should not be interpreted as simple evidence that one immune pathway is responsible. Reduced lymphoid cells can influence host defense, inflammation, immune tolerance, and responses to treatment, while later repopulation may alter those effects. Tracking depletion alongside its timing therefore provides context for separating early consequences from recovery-associated changes.