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JoVE Journal
Immunology and Infection

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Depletion and Reconstitution of Macrophages in Mice
 

Depletion and Reconstitution of Macrophages in Mice

Article DOI: 10.3791/4105
August 1st, 2012

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Summary August 1st, 2012

Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.

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