Mouse Brain Tissue
Mouse Brain Tissue - Related Videos
02:37
Frozen Mouse Brain Tissue Sectioning: A Procedure to Obtain Thin Frozen Tissue Sections from Frozen Murine Brain Tissue
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2023
In this video, we demonstrate the sectioning of a frozen mouse brain tumor tissue using a cryostat. The frozen brain tissue sections so obtained are stored at low temperatures until further analysis.
08:47
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Cited by 52 •
2010
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
02:51
Measuring the Viscoelastic Properties of Mouse Brain Tissue Using Rheometry
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2025
Source: Canovic, E. P., et al. Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry. J. Vis. Exp. (2016)In this video, a rheometer was used to apply controlled oscillatory rotational motion to brain tissue to generate shear strain and evaluate the tissue's viscous and elastic properties. The rheometer, as a functional tool, it provides insights into brain mechanics during injury or neurodegenerative diseases.
03:47
Obtaining Cell Suspensions from Ischemic Mouse Brain Tissues
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2025
This video demonstrates the procedure for isolating brain cells and infiltrated immune cells from ischemic mouse brain tissues by enzymatic digestion, followed by the removal of cell debris and myelin by density gradient centrifugation, to study inflammation responses in stroke research.
04:46
Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section
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2025
This video demonstrates the procedure for immunostaining a fixed mouse brain tissue section with fluorescently labeled astrocytes using primary and secondary antibodies and capture the images of the stained astrocytes through confocal microscopy.
FAQs
Neurons transmit information through electrical impulses and chemical synapses, making them central to neural communication. Glial cells instead regulate the surrounding cellular environment, provide metabolic support, and influence signaling between neurons. Examining both populations helps researchers connect changes in cellular support or communication with differences in neural function and behavior.
Blood vessels and extracellular structures provide essential context for interpreting neural organization. Vessels are part of the tissue environment, while extracellular structures help support the arrangement and interactions of cells. Including these components allows studies to examine brain tissue as an organized biological system rather than viewing neuronal activity or molecular changes in isolation.
Measurements of gene and protein expression can show how biological processes change across brain regions, developmental stages, or experimental conditions. When paired with information about cellular organization and neural circuits, these molecular findings help relate altered expression to neural function, responses to injury or disease, and mechanisms that may be relevant to therapeutic research.
Researchers can examine tissue sections, cultured samples, or freshly isolated brain regions, with each format providing a different perspective. Sections preserve spatial organization for studying anatomy and cell distribution, cultures support examination of cellular behavior under controlled conditions, and freshly isolated regions retain material suitable for analyzing region-specific biological features and molecular changes.
This model is useful when investigators need to connect brain structure with changing biological function. Tissue-based analyses can map neuroanatomy, examine how neural circuits are organized, and follow developmental changes in cells or molecular expression. These approaches support studies linking cellular events to information processing, behavior, and the maturation of neural systems.
Comparing tissue from different biological conditions can reveal cellular organization, gene or protein expression, and other molecular changes associated with injury or disease. Such findings help identify affected processes and provide evidence for evaluating potential therapeutic approaches. The tissue model therefore connects observed neurological changes with underlying biological mechanisms rather than relying only on behavioral outcomes.