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Neuroscience

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Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
 

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

Article DOI: 10.3791/2021
July 20th, 2010

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Summary July 20th, 2010

We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.

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