Non-target Control
Non-target Control - Related Videos
06:43
Esophageal Heat Transfer for Patient Temperature Control and Targeted Temperature Management
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Cited by 6 •
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This study presents a novel method to provide efficient patient temperature control for cooling or warming patients. A single use, triple lumen device is placed into the esophagus, analogous to a standard orogastric tube, and connects to existing heat exchange units to perform automatic patient temperature management.
07:54
Drug-Induced Sleep Endoscopy (DISE) with Target Controlled Infusion (TCI) and Bispectral Analysis in Obstructive Sleep Apnea
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Cited by 16 •
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The aim of this study was to establish a standardized protocol for sleep endoscopy to differentiate obstruction patterns in obstructive sleep apnea (OSA). Target-controlled infusion (TCI) of the sedative was combined with real-time monitoring of the depth of sedation using bispectral analysis.
03:26
Targeted Muscle Reinnervation: Surgical Protocol for a Randomized Controlled Trial in Postamputation Pain
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Cited by 5 •
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The protocol outlines the surgical procedure for the treatment of postamputation pain using Targeted Muscle Reinnervation (TMR). TMR will be compared with two other surgical techniques, specifically Regenerative Peripheral Nerve Interface (RPNI) and neuroma excision, followed by immediate burying within muscle under the context of an international, randomized controlled trial.
08:40
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Cited by 1 •
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The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.
08:45
An Introduction to Motor Control
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2023
Motor control involves integration and processing of sensory information by our nervous system, followed by a response through our skeletal system to perform a voluntary or involuntary action. It is vital to understand how our neuroskeletal system controls motor behavior in order to evaluate injuries pertaining to general movement, reflexes, and coordination. An improved understanding of motor control will help behavioral neuroscientists in developing useful tools to treat motor disorders, such...
FAQs
Matching the reagent’s format, concentration, and delivery conditions makes the comparison more controlled. If the target-specific treatment produces an effect that the Non-target Control does not, the difference is more plausibly associated with the intended target interaction rather than unequal exposure or handling. This design therefore improves attribution of observed cellular or molecular changes.
A scrambled or non-complementary nucleic acid sequence is useful because it preserves the presence of a nucleic-acid reagent without intentionally binding the target transcript. Effects appearing in both the sequence-specific treatment and this control can therefore signal sequence-independent responses, whereas effects unique to the target treatment provide stronger evidence for target-dependent activity.
Comparison with a Non-target Control helps separate several confounding influences: transfection-related changes, reagent toxicity, sequence-independent responses, and routine manipulation. These influences may alter cells even when the intended target is not affected. Treating the control as the experimental baseline allows researchers to judge whether a measured phenotype reflects target engagement or an artifact of the treatment process.
An appropriate workflow applies the control and target-specific reagent under the same experimental handling, using matched format, concentration, and delivery conditions. Researchers then compare the resulting samples for the biological or molecular readout of interest. Keeping these parameters aligned is essential because differences in preparation or exposure could be mistaken for effects caused by target-specific activity.
Researchers use this control most directly in gene-silencing experiments, where a target-specific nucleic acid is delivered alongside a sequence-matched reagent that should not specifically bind the target transcript. The approach is relevant across cell biology, molecular biology, and functional genomics, particularly when interpreting whether a change reflects the intended silencing design or the experimental manipulation itself.
If the target-treated sample changes while the Non-target Control remains comparatively unchanged, the result supports a target-dependent interpretation. If both conditions change similarly, investigators should consider transfection, toxicity, sequence-independent responses, or routine handling as possible explanations for the observed outcome. This comparison strengthens conclusions about whether the measured phenotype is linked to the intended target.